| tv | What's in the Box? (2025)As Self - Host(6 episodes) | (Not seen) |
| tv | Uncoupled (2022)As Michael Lawson(8 episodes) | (Not seen) |
| movie | The Unbearable Weight of Massive Talent (2022)As Richard Fink | (Not seen) |
| tv | It's a Sin (2021)As Henry Coltrane(1 episode) | (Not seen) |
| movie | 8-Bit Christmas (2021)As Adult Jake Doyle / Narrator | (Not seen) |
| movie | The Matrix Resurrections (2021)As The Analyst | (Not seen) |
| tv | Home Movie: The Princess Bride (2020)As Westley(1 episode) | (Not seen) |
| tv | Under the Power (2019) (AKA "锦衣之下")As Travis Cleveland | (Not seen) |
| tv | A Series of Unfortunate Events (2017)As Count Olaf(25 episodes) | (Not seen) |
| movie | Downsizing (2017)As Jeff Lonowski | (Not seen) |
| tv | Hot Ones (2015)As Self(1 episode) | (Not seen) |
| tv | The Tonight Show Starring Jimmy Fallon (2014)As Self(1 episode) | (Not seen) |
| tv | Late Night with Seth Meyers (2014)As Self(2 episodes) | (Not seen) |
| movie | Gone Girl (2014)As Desi Collins | (Not seen) |
| movie | A Million Ways to Die in the West (2014)As Foy | (Not seen) |
| movie | Cloudy with a Chance of Meatballs 2 (2013)As Steve (voice) | (Not seen) |
| movie | The Smurfs 2 (2013)As Patrick Winslow | (Not seen) |
| movie | American Reunion (2012) (AKA "American Pie: Reunion")As Celebrity Dance-Off Host | (Not seen) |
| tv | American Horror Story (2011)As Chester Creb(2 episodes) | (Not seen) |
| movie | The Smurfs (2011)As Patrick Winslow | (Not seen) |
| movie | A Very Harold & Kumar Christmas (2011)As Neil | (Not seen) |
| movie | The Muppets (2011)As Self | (Not seen) |
| tv | Adventure Time (2010)As Prince Gumball (voice)(3 episodes) | (Not seen) |
| movie | Cats & Dogs: The Revenge of Kitty Galore (2010)As Lou (voice) | (Not seen) |
| tv | RuPaul's Drag Race (2009)As Self - Guest Judge(1 episode) | (Not seen) |
| tv | Glee (2009)As Bryan Ryan(1 episode) | (Not seen) |
| movie | Cloudy with a Chance of Meatballs (2009)As Steve the Monkey (voice) | (Not seen) |
| tv | Dr. Horrible's Sing-Along Blog (2008)As Dr. Horrible(3 episodes) | (Not seen) |
| movie | Dr. Horrible's Sing-Along Blog (2008)As Doctor Horrible | (Not seen) |
| movie | Justice League: The New Frontier (2008)As Barry Allen / The Flash (voice) | (Not seen) |
| movie | Harold & Kumar Escape from Guantanamo Bay (2008) (AKA "Harold & Kumar 2")As Neil Patrick Harris | (Not seen) |
| tv | The Graham Norton Show (2007)As Self(1 episode) | (Not seen) |
| tv | Robot Chicken (2005)As Executive / Harvey Dent / Two-Face / Three-Face / Four-Face (voice)(1 episode) | (Not seen) |
| tv | How I Met Your Mother (2005)As Barney Stinson(208 episodes) | (Not seen) |
| movie | Serenity (2005)As Screaming Reaver (uncredited) | (Not seen) |
| movie | Harold & Kumar Go to White Castle (2004) (AKA "Harold & Kumar Get the Munchies")As Neil Patrick Harris | (Not seen) |
| tv | The Ellen DeGeneres Show (2003)As Self(2 episodes) | (Not seen) |
| movie | Undercover Brother (2002)As Lance | (Not seen) |
| movie | The Next Best Thing (2000)As David | (Not seen) |
| tv | Family Guy (1999)As Barney Stinson (voice)(2 episodes) | (Not seen) |
| tv | Will & Grace (1998)As Bill(1 episode) | (Not seen) |
| movie | The Christmas Wish (1998)As Wil Martin | (Not seen) |
| movie | The Proposition (1998) (AKA "Shakespeare's Sister")As Roger Martin | (Not seen) |
| movie | Starship Troopers (1997)As Colonel Carl Jenkins | (Not seen) |
| tv | The Daily Show (1996)As Self(3 episodes) | (Not seen) |
| tv | Homicide: Life on the Street (1993)As Alan Schack(1 episode) | (Not seen) |
| tv | Doogie Howser, M.D. (1989)As Doogie Howser(97 episodes) | (Not seen) |
| tv | The Simpsons (1989)As Neil Patrick Harris (voice)(1 episode) | (Not seen) |
| tv | Saturday Night Live (1975)As Self - Host(1 episode) | (Not seen) |
| tv | The Oscars (1953)As Host(1 episode) | (Not seen) |